KaLy-Cell conducts in vitro pre-clinical metabolism studies with xenobiotics using hepatocytes, freshly isolated or cryopreserved, from various species.

Metabolic Stability and Identification

Metabolic stability influences both oral bioavailability and plasma half-life of a compound, which in turn, affect its efficacy. KaLy-Cell evaluates the metabolic stability of drug candidates in microsomes or hepatocytes from various species in suspension.

Our contract service assesses the stability of the compound relating to biotransformation by measuring the disappearance of parent compound in the selected test system.

  • LC/MS method development for analysis of test article  

  • Incubations of microsomes or hepatocytes (fresh or cryopreserved) in suspension under continuous shaking, and measured at multiple time-points.

  • Incubations of marker substrates as positive controls to check for metabolic competency of the test system (positive controls quantified by formation of metabolites)

  • Full data summary

In Vitro Species Comparison

  • Cytochrome P450 metabolism can vary widely from one species to another, so determining the best animal model is important for pharmacokinetic and toxicity studies.

  • Species differences in drug metabolism can be assessed by studying the in vitro metabolism of a drug by hepatocytes from a variety of species of laboratory animals (e.g., monkey, dog, rabbit and rat). This analysis compares metabolic stability profiles from humans and animal species in order to identify the most relevant animal model for pharmacokinetic and toxicity studies.

  • We offer species comparison as a contract service, and we offer products for customers who perform their own in-house comparisons.

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